Journal: Microorganisms
Article Title: Clostridioides difficile Infections in Children: What Is the Optimal Laboratory Diagnostic Method?
doi: 10.3390/microorganisms12091785
Figure Lengend Snippet: PPV vs. NPV comparison for all methods & algorithms in 2–18 years old. CAC, CHROMagar C. difficile ; CDQ, C. diff Quik Chek Complete kit immunoassay; GDH, Glutamate dehydrogenase; NPV, Negative predictive value; PPV, Positive predictive value; QGP, QIAstat-Dx Gastrointestinal Panel 2 PCR test; XCD, Xpert C. difficile PCR test. Algorithm 1, CDQ arbitrated by XCD; Algorithm 2, CDQ arbitrated by QIAstat-Dx Multiplex PCR; Algorithm 3, XCD followed by toxin A/B from the CDQ kit; Algorithm 4, QGP followed by toxin A/B from the CDQ kit.
Article Snippet: All consecutive samples were tested prospectively for the presence of toxins A and B and GDH using the C. diff Quik Chek Complete kit immunoassay (CDQ; Techlab, Blacksburg, VA, USA), detection of toxin A and B genes using the QIAstat-Dx Gastrointestinal Panel 2 PCR test (QGP; QIAGEN, Hilden, Germany), and detection of toxin B gene using the Xpert C. difficile PCR test (XCD; Cepheid, Sunnyvale, CA, USA).
Techniques: Comparison, Diff-Quik, Multiplex Assay